Pine woods littersnakes are solitary, terrestrial snakes that are most active during the daytime and evening hours in warmer months. They can be found under rocks, fallen bark, and debris on the forest floor, hunting for prey. Pine littersnakes will occasionally climb into low shrubs and bushes. When threatened, they may flatten their bodies and raise their tails as a defense posture. If further provoked, pine littersnakes will quickly flee into leaf litter or underground burrows.
These docile snakes prefer cooler, moist microhabitats during the hot Florida summers. Extended droughts can impact local populations. Pine littersnakes brumate underground during winter months. They emerge in early spring to bask and become active again through late fall.